Sabine Flitsch presented her efforts in generating IM-MS fragment libraries in order to characterise alpha/beta configuration of glycans and its application of determining the specificity of desialylating enzymes as well as the characterisation of natural glycosides which have been extended from amygdalin.
Larah Mahal described a lectin platform and miRNA proxy platform for the role of core fucosylation and FUT8 that is upregulated in melanoma metastasis. This affects the cleavage of adhesion molecule L1CAM and thus shaping cell migration.
Hiren Joshi introduced the term Glycotopiary which is a simple cell technology to address the glycosylation of O-linked, N-linked and heparan sulphate. Examples included the discovery of new O-mannosyl transferases and the engineering of adhesins that selectively binds muc7 and gp1ba, but not other mucins. The software tool Glycodomainviewer was presented as a way to capture and display sites of glycosylation on glycoproteins
Ola Blixt presented the development and targeting of single chain antibodies to O-linked and advanced glycation end products, including the antibody scfv g2d11 that binds bis Tn antigen in gastric mucosa and scfv D1-B2 against AGE, staining atherosclerotic plaques and pancreatic cancer.
Erdmann Rapp has identified the lack of useful tools for the analysis of MS-deteced glycopeptides and introduced glyXtoolsMS based on open ms c++. In practice, he and demonstrated its applicability on tandem data from IgG and fibrinogen
Frédérique Lisacek introduced the glycomic tab on Expasy and associated modular software including Pepsweetener, calculator, Glynsight, Glycosite align, Glydin and Glyconnect
Will York asked the question of how to navigate around the glyco-data and introduced a conceptual schema to visualise data based on RDF. His vision was a bioinformatics infrastructure based on the NIH R34 Grant, unifying the current resources in glyco-bioinformatics and databases
Kerry Gilmore presented the automated glyco- assembly line, and the use of chemistry and reaction conditions to control stereochemistry to produce designed oligosaccharides.
Ten Feizi showed the development of an O-glycan array and demonstrated its usability to Rotavirus p(10) and p(19) interaction with porcine gastric mucin oligosaccharides and in particular the linear H type 1 structure.
Manfred Wuhrer discussed how to increase throughput for glycomics for and clinical relevance (colorectal cancer and MALDI imaging) and compared his findings applying different platforms (MALDI-MS, HILIC-HPLC and xCGE-LIF)
Gerald Hart updated us about the O-GlcNacylation and its involvement in nutrient regulation and transcriptional regulation, including O-GlcNAcylation of the tata binding protein (tbp), where the glycosylation of T114 impairs metabolic gene expression.
Matthew Campbell presented the development a repository, called Glycostore that stores analysis data from HLIC, PGC and CE analysis including retention times for N-glycans, O-glycans, glycolipids. In addition, he shows interconnections with other glycoresources using GlycoRDF ontology
The talks were concluded by the presentation by Will York about the MIRAGE project , what it is, and how we should endorse the use of the guidelines. Finally, a panel talked about the term “Systems Glycomics”. The panelists discussed both in terms how it can specifically identify the glycosylation as part of a systems biology approach as well as providing a “buzz word” that will help in providing attention to glycosylation for decision makers and funding agencies.