1. Publication standards for interaction analysis data
Interaction analysis includes experiments which seek to identify and define the recognition of glycan receptors by binding partner proteins. These interactions may be as limited as the interaction of an individual proteins with a single glycan or as broad as the evaluation of whole binding of cells to populations of glycans.
Initial efforts focus on the interactions that can be evaluated on a glycan microarray. Future expansion would include all types of interaction experiments, including, but not limited to: SPR (surface plasmon resonance), flow cytometry, ELISA, STD-NMR and ITC (isothermal calorimetry).
The aims of the MIRAGE working group “Interaction Analysis” are:
- define and provide for interaction experiments, e.g.,
- sample reporting guidelines
- experimental guidelines
- platform guidelines
- results guidelines
- provide templates for reporting within the above guidelines
- suggest guidelines for data reporting (normalization standards)
- conventions for glycan nomenclature in order to facilitate cross platform comparisons of different glycan arrays
- interact with the MIRAGE working group “Bioinformatics” to establish basic structure for data reporting
- recruit users/experts in other platforms to review/critique guidelines for use in those experiments